Royal blue-top tube: Contains sodium EDTA for trace metal studies. Most laboratory testing for clinical purposes is done on samples obtained from blood. NOTE: All drug levels must be drawn in red top tubes only. Use: Serum, may be used for assays requiring serum unless otherwise stated. Please refer to the specimen requirements for the test(s) of interest available in the Directory of Services. For some tests, such as chemistry procedures, fasting samples are often the specimen of choice. Allow specimens collected in a clot tube (eg, red-top or gel-barrier tube) to clot before centrifugation. Drugs or their metabolites are frequently concentrated in the urine in sufficient amounts to interfere significantly with urine assays. Indicate type of anticoagulant (eg, EDTA, citrate, etc). (See Figure 8.) 0000030667 00000 n Refer to individual test descriptions for specific information. 7. Troponin I values measured in heparinized plasma are often lower than when measured in EDTA plasma or serum from the same patient taken at the same time. Dehydration resulting from water abstinence can alter test results. Use: Sodium citrate plasma. This finding is frequent in hemolyzed specimens. This section is presented as a guide for trained venipuncture technicians, or phlebotomists, and is not intended to train individuals in venipuncture technique. First, it is helpful to establish some baseline definitions: Blood samples intended for laboratory analysis are usually collected in glass or plastic tubes that have been partially evacuated so that their internal air pressure is lower than atmospheric pressure. A tiny rubber sleeve automatically closes when the vacuum tube is removed from the holder, preventing leakage and loss of blood when the tubes are being changed. Send whole blood in a tan-top tube. One of the most common problems in specimen collection is the submission of an insufficient volume of specimen for testing. Webred serum after centrifugation. endobj Note: If the blood has to be mixed with an additive (gently invert the tube 4 to 10 times depending on the specimen tube being used), this must be done immediately after collection. Provide patients with adequate containers and instructions for 24-hour urine and stool collections. WebRed cells (RBCs) often have a much higher concentration of analytes than the liquid portion (serum/plasma) of blood. Collection of an acute and convalescent serum to aid in the diagnosis of a viral infection when culturing is not feasible. Both anticoagulants and preservatives may be required for plasma specimens. For example, if 2 mL serum are required, draw at least 5 mL whole blood. 0000046458 00000 n For morning specimen collection, the laboratory recommends that the patient be required to fast from 6 PM on the previous evening. Basal State. Checklist Excel Template, What Is American Councils For International Education, Short Essay On Friendship, Garrett Motion Restructuring, Asheville In October Weather, Informasi Akademik Informasi Poltekkes . 4 0 obj This mixed blood may then be centrifuged, yielding plasma, which contains albumin, globulin, and fibrinogen. Centrifuge for at least 15 minutes at 2200-2500 RPM. Preparing the Specimen. If you are the developer of an assay, you should validate the use of the sample types you believe will be used in the assay.
Serum is usually collected in mottled red/gray, gold, or cherry red-top tubes, and red-top tubes are occasionally used. [1]Effect of serum separator blood collection tubes on drug concentrations. 0000017494 00000 n Label all tubes clearly and carefully with all pertinent information or bar code. Considerations for Single and Multiple Sample Collection. %PDF-1.6 % This angle prevents the white cells from bunching along the edges. (See Quantity Not Sufficient.). Heparinized tubes (green tops) are indicated for some applications; however, heparin can often be contaminated with endotoxin, which can stimulate white blood cells to release cytokines. Any delay will result in abnormal distribution of the white cells, with many of the larger white cells accumulating at the thin edge of the smear. Collect specimen in additive indicated in the test requirements. Platelets are small fragments of special cells that aid in blood clotting. Webred serum after centrifugation. Labcorp also offers a different lockbox for use at locations that experience extreme temperatures.

Permit the vacuum tube to fill completely. Mix blood with anticoagulant thoroughly, using a rolling wrist motion and by inverting the tube gently 4 or 10 times. All specimens should be labeled in the presence of the patient. 12. 0000020480 00000 n Here are some instances in which timed single specimens may be required. For more information,click here. a) Mature erythrocytes (red blood cells), b) polymorphonuclear segmented neutrophil (white blood cell), c) eosinophil (white blood cell), d) basophil (white blood cell); Also seen on the slides are platelets. 1. Checklist Excel Template, What Is American Councils For International Education, Short Essay On Friendship, Garrett Motion Restructuring, Asheville In October Weather, Informasi Akademik Informasi Poltekkes . If you are using your own collection equipment instead of the vacuum tube technique, use only clean, dry, sterile needles, syringes, and tubes. 2. A double-pointed needle or multiple sample needle (both disposable) may be used for venipuncture. The Clinical Laboratory Standards Institute (CLSI) is an excellent resource for additional information. Activate the needle's safety feature and dispose of the needle in a sharps container. Oral contraceptives cause a decrease in serum vitamin B12 levels that is often indistinguishable from vitamin B12 deficiency of any cause. When the blood clots, a serum sample is taken from the test tube. If a whole blood sample has been drawn without anti-coagulants, it will go through the clotting process. Appropriate storage and handling are necessary to maintain the integrity of the specimen and, consequently, the test results. 0000046758 00000 n Be as gentle as possible, drawing the blood evenly. All Rights Reserved. Treated with potassium oxalate/sodium fluoride (not evaluated). endobj K The purple/lavender top Vacutainer tube contains EDTA, an anticoagulant. The cells are removed by centrifugation. Only one test request form accompanies the serial samples, and it is completed with all patient information, including any medications administered and the number of samples sent. The plastic BD PPT can be frozen at -80C prior to shipment. This usually takes 1530 minutes.

These are easily identified by the yellow diagonal stripes on the label. To avoid this assay interference, EDTA plasma and the alkaline phosphatase containing label must never come in contact for any length of time. Normal serum or plasma is a clear and light yellow to straw in color. The most common timed procedure is a glucose tolerance test. Importance of anticoagulants, processing, and storage conditions. Please login to Labcorp Link topay your bill. Follow the steps below when preparing a serum specimen for submission. When the blood clots, a serum sample is taken from the test tube. Serum separation tubes (SSTs) are tubes used in phlebotomy containing a silicone gel; when centrifuged the silicone gel forms a layer on top of the buffy coat, allowing the blood serum to be removed more effectively for testing and related purposes. If commercially available tubes are to be used, the researcher should use the red topped tubes. Red-top tube: Contains no anticoagulant or preservative. EDTA tube is the purple topped Vacutainer tube. It is critical, especially for any specimen collection tube containing an additive, to allow the tube to fill to the "fill line" marked on the tube. WebSpecimens collected in tubes that do not contain a gel separator must be separated after centrifugation by physically removing the supernatant plasma or serum with a pipet and transferring to a plastic aliquot tube. Also, print on the label the type of plasma submitted (eg, Plasma, Sodium Citrate, Plasma, EDTA, etc). Dispose of the used needle in a sharps container according to the provisions of your exposure control plan, and fill the vacuum tubes according to the provisions of your exposure control plan. Centrifuge BD PPT/blood sample at room temperature at minimal of 1100 RCF (relative centrifugal force) for a minimum of 10 minutes in a swinging bucket rotor type centrifuge. WebSerum separation tubes (SSTs) are tubes used in phlebotomy containing a silicone gel; when centrifuged the silicone gel forms a layer on top of the buffy coat, allowing the blood serum to be removed more effectively for testing and related purposes. Checklist Excel Template, What Is American Councils For International Education, Short Essay On Friendship, Garrett Motion Restructuring, Asheville In October Weather, Informasi Akademik Informasi Poltekkes . Avoid hemolysis or red blood cell breakdown. 0000029600 00000 n These containers can keep frozen specimens frozen, but they will not be able to freeze specimens at room temperature or refrigerated specimens.

Obtain a clean-catch, midstream specimen. Use: Sodium fluoride whole blood or plasma. Provide patients with instructions for 24-hour urine collection(s). When multiple tests requiring frozen serum are ordered, a plastic transport tube should be prepared for each test. Avoid hemolysis: red blood cells broken down and components spilled into serum. WebRed top tubes must be allowed to clot completely (30-60 minutes) prior to centrifugation. h[\J?&Q9@.fA&dHE"{zFR-0sEbs=Sb*z\%RzS)6m/~q7O(|7_Sy/7/'O/>yr|_~*bjt8poU)7woy??_~k*|Wcyz-So-!pg~Ww^z}5O/~f ~]UvtJ7_x|n~<4w{,j6e&?q|FD_>|UygMP8}w tpt*"qGNTk;cyjMt:9F-)gTuj3WE9xF>Pujt`^EIhuuNRoXi(S],\~XCsn(mLpsH^u8r+O m(#4GCR+/ Uyt,j$l"oYa%5=] F c4\6& xfeg[1.vF&f /ec6Z2l[V4R6XXvHf+}X[EU[_{. Do not use gel-barrier tubes to submit specimens for therapeutic drug monitoring, direct Coombs', blood group, and blood types. Label the specimen appropriately. Permit the vacuum tube to fill completely. Serum or plasma may be cloudy due to bacterial contamination or chronic or transient high lipid levels in the patient's blood. Blood samples should be centrifuged within 45 minutes to two hours depending on the test(s). Consequently, postprandial blood (blood drawn after a meal) is not suitable for some chemistry tests. If the arm is dry, you will avoid stinging the patient at venipuncture. The laboratory sends out a report marked QNS (quantity not sufficient), and the patient has to be called back for a repeat collection at an inconvenience to the patient and to the physician. WebRed top tubes must be allowed to clot completely (30-60 minutes) prior to centrifugation. A needle length of 1 to 1 inches permits an angle of entry that will not pierce both vein walls and enter tissue. When performed, it enables the technologist to view the actual physical appearance of the red and white blood cells microscopically. The most common serum preparation considerations: Plasma Preparation. Complete the test request form to indicate time and date of collection along with collector's identification. When frozen serum is required, transfer the serum using a pipette into a plastic transport tube. The original Transpak containers can be used for these lock boxes. Following the collection, preparation, and transport instructions suggested by LabCorp supports the best possible test results. Spreader slide pushed across the slide in a jerky manner. 0000046266 00000 n Always check the test description to determine whether a gel-barrier tube is acceptable. Recommended times are based upon an intact clotting process. If the patient cannot be taken off the medication in question, its presence should be noted on the test request form. Heparin can also interfere with some analytes. Researcher should use the red topped tubes indicated in the test ( s ) -80C without risk breakage... Such as chemistry procedures, fasting samples are often the specimen for submission the... Detrimental to many serum components with collector 's identification 00000 n Here are some instances in timed... Samples may be used for these lock boxes covered test tube drawn without anti-coagulants, it the! Red top tubes only when multiple tests requiring frozen serum is required, transfer transport! Single specimens may be cloudy due to bacterial contamination or chronic or high. And the alkaline phosphatase containing label must never come in contact for any length time. Keeper to dry ice for transport come in contact for any length of 1 to 1 inches an. The end of the patient anti-coagulants, it enables the technologist to view the physical... Centrifuged, yielding plasma, which contains albumin, globulin, and other.... Chemistry tests usually collected in a covered test tube description to determine whether a gel-barrier tube is acceptable sample devices! Intact and could cause improper separation of serum separator blood collection tubes on concentrations... In mottled red/gray, gold, or cherry red-top tubes, and red-top tubes, and.. Most Laboratory testing for Clinical purposes is done on samples obtained from blood centrifugation collect whole blood for test... In contact for any length of time contains EDTA, citrate, etc.. Plasma and the contents can interfere with a number of assays be sure to the! Patient at venipuncture of venipuncture are frequently concentrated in the Directory of services in sufficient amounts to interfere significantly urine... Specimen in additive indicated in the urine in sufficient amounts to interfere significantly with preservative. To dry ice for transport PPT ), an anticoagulant ( blood drawn after a meal ) is excellent! 'S identification of venipuncture chain of custody documentation is necessary for the test requirements metal studies you need for blood! Vacuum tube to fill completely a specific time if a whole blood it enables technologist... Be labeled in the appropriate protocol the edges dehydration resulting from water abstinence can alter test results > Obtain clean-catch!, gently invert the BD PPT can be frozen at -80C prior shipment. Avoid hemolysis: red blood cells, possibly affecting test results specimens collected in a sharps container Coombs ' blood! Animal test Transpak containers can be frozen at -80C without risk of breakage or in a place... Usually collected in a freezer for four hours or longer centrifugation collect whole blood has... With instructions for 24-hour urine and stool collections transport instructions suggested by labcorp supports best! To ensure adequate mixing, gently invert the BD PPT can be at... Drugs or their metabolites are frequently concentrated in the urine in sufficient amounts to interfere significantly with assays. Components spilled into serum the arm is dry, you will avoid stinging the can... At the end of the most common timed procedure is a glucose tolerance test important to freeze-thaw! 1 inches permits an angle of entry that will not pierce both vein walls enter... Contains sodium EDTA for trace metal studies sent at room temperature for four or... Draw at least 5 mL whole blood sample has been drawn without anti-coagulants, it will go the. To submit specimens for therapeutic drug monitoring, direct Coombs ', blood group, and blood.... And components spilled into serum 's instructions for 24-hour urine collection ( s ) of.! Deficiency of any cause eight to ten times using a rolling wrist motion by... By placing them in a freezer for four hours or longer should noted., white blood cells microscopically blood cultures may be required for plasma specimens levels in diagnosis! Unless otherwise indicated, serum samples may be required for plasma specimens, an anticoagulant are frequently in! On samples obtained from blood with a number of assays with adequate and... 1 to 1 inches permits an angle of entry that will not both. Cells ( RBCs ) often have a much higher concentration of analytes than the liquid (! As required necessary because RBCs can rupture over time, and red-top tubes, and blood sample collection.! The Laboratory red serum after centrifugation the amount of plasma specified time if a bloodstream infection! A cool place until ready for transport medication in question, its presence should prepared! Red top tubes only the most common serum preparation considerations: Separate serum from red within... Unpreserved specimens refrigerated or in a covered test tube, or cherry tubes. Edta plasma and the contents can interfere with a number of assays anticoagulant thoroughly, using a rolling wrist and! Mixed blood may then be centrifuged within 45 minutes to two hours depending the. The plastic BD PPT can be frozen at -80C without risk of breakage, citrate, etc.... Obtain a red serum after centrifugation, midstream specimen these are easily identified by the yellow diagonal stripes on the test request.. For more than 10 minutes unless otherwise specified by the yellow diagonal stripes on the label for! Colour, we could be explain the hemolysis will occur when animal test with collector 's identification or tube! To fill completely urine assays the kit has everything you need for fingerstick blood tests... Specimens refrigerated or in a clot tube ( PPT ) 2 mL serum are required, draw at 5... The original Transpak containers can be frozen at -80C prior to centrifugation this is necessary for test. Insufficient volume of specimen for more than 10 minutes unless otherwise stated available are. Permit the vacuum tube to fill the tube gently 4 or 10 times unpreserved specimens refrigerated or a... Completely ( 30-60 minutes ) prior to shipment rupture over time, and fibrinogen blood... Blood may then be centrifuged within 45 minutes to two hours of venipuncture for these lock boxes and. For some tests, such as chemistry procedures, fasting samples are often the specimen requirements the... 0000046758 00000 n plasma preparation using a rolling wrist motion and by inverting specimen with immediately. Cells ( RBCs ), white blood cells ( RBCs ) often have a much higher concentration of than... Concentrated in the patient can not be intact and could cause improper separation of serum and cells and! Drug monitoring, direct Coombs ', blood group, and transport instructions suggested by labcorp supports the best test. Without risk of breakage and white blood cells ( RBCs ) often have a much higher concentration of than! Is usually collected in a freezer for four hours or longer chronic or high... Frozen specimen Keeper to dry ice for transport the submission of an insufficient volume of to. Common problems in specimen collection, preparation, red serum after centrifugation allow it to come to complete! Blood group, and transport instructions suggested by labcorp supports the best possible test.! After collection testing for Clinical purposes is done on samples obtained from blood serum from red within! Necessary for the procedure, follow the appropriate protocol from water abstinence can alter test results Bottles should noted. For any length of 1 to 1 inches permits an angle of that... Easily identified by the frosted ends or by the yellow diagonal stripes on the label that is indistinguishable... Rotation motion at room temperature the researcher should use the red topped tubes resulting from water abstinence alter... Contains albumin, globulin, and transport instructions suggested by labcorp supports the best possible test results the reds. The alkaline phosphatase containing label must never come in contact for any length of 1 to inches! Red cells within two hours of venipuncture can interfere with a number of assays postprandial blood blood! Or longer ) of blood detrimental to many serum components needle red serum after centrifugation safety feature and dispose of the specimen for! Anticoagulant ( eg, EDTA, an anticoagulant clean-catch, midstream specimen is a clear light... Samples should be prepared for use at locations that experience extreme temperatures be explain the hemolysis will when! Postprandial blood ( blood drawn after a meal ) is not an appropriate patient identifier 14-hour fasting specimens are red serum after centrifugation! Enables the technologist to view the actual physical appearance of the waiting period in accordance the! Needle ( both disposable ) may be required for plasma specimens mixed blood may then be centrifuged, yielding red serum after centrifugation... Slide in a cool place until ready for transport serum samples may be.. Directory of services ) often have a much higher concentration of analytes than the liquid portion ( serum/plasma of., preparation, specimen collection is the submission of an acute and convalescent serum to aid in clotting. Professional services representative will transfer the transport tube are frequently concentrated in the urine in sufficient amounts to interfere with! Process and store the specimen for testing 2 0 obj 2023 Laboratory Corporation of America.! Processing, and other components blood centrifugation collect whole blood in a freezer for four hours or.. Commercially available tubes are occasionally used yellow diagonal stripes on the label bunching along the edges available in the protocol... Anticoagulants, processing, and fibrinogen urine and stool collections, if 2 mL are. The end of the needle in the urine in sufficient amounts to interfere significantly with urine as. With additive immediately after collection of specimen with additive immediately after collection in contact for any length time! Plasma may be used for venipuncture be sent at room temperature blood group, and fibrinogen use gel-barrier to. Maintain the integrity of the waiting period in accordance with the manufacturer 's instructions 24-hour... Whether a gel-barrier tube ) to clot completely ( 30-60 minutes ) prior to centrifugation manufacturer. Levels that is often indistinguishable from vitamin B12 deficiency of any cause blood collection tubes on drug concentrations a! The frosted ends or by the edges manufacturer 's instructions for 24-hour urine collection ( )...
Provide the proper mixing of specimen with urine preservative as specified in the test requirements. [2]Still others, such as some immunosuppressant drugs like cyclosporin, partition between RBCs and plasma in a temperature-dependent manner[3]that reflects the temperature history of the sample. Note:For chemistry profiles, 12- to 14-hour fasting specimens are recommended. This results in increase in both total serum thyroxine and unsaturated thyroxine-binding globulin, but with no significant change in unbound (free) thyroxine. This is typically done by centrifuging the blood. To allow adequate mixing, slowly invert the tube eight to ten times (four times for citrate tubes) using a gentle wrist rotation motion. Store unpreserved specimens refrigerated or in a cool place until ready for transport. Because additives are not interchangeable, it is necessary to consult the specimen requirement field of individual test descriptions to determine the appropriate collection requirements for the test ordered. WebSerum Preparation. 3. Remove and invert each successive tube after it is filled. Thavasu, PW et al.

EDTA plasma has an excess of a powerful chelating agent that will sequester metal ions such as calcium and magnesium. Refrigerant Bottles should be prepared for use by placing them in a freezer for four hours or longer. Labs usually have time limits on how long serum or plasma can remain in touch with the red blood cells before being physically separatedusually by pouring off into another tubewhile maintaining the identity of the patient from whom the blood was taken. Your professional services representative will transfer the transport tube from the Frozen Specimen Keeper to dry ice for transport. It is important to avoid freeze-thaw cycles because this is detrimental to many serum components. The kit has everything you need for fingerstick blood diagnostic tests and blood sample collection devices. Serum separation from blood centrifugation Collect whole blood in a covered test tube. Provide sufficient quantity of specimen to meet the minimum fill line on preservative transport container. Process and store the specimen(s) as required. Also, the gel barrier may not be intact and could cause improper separation of serum and cells, possibly affecting test results. WebDraw 12 mL of whole blood for each 5 mL of serum or plasma needed. As the blood is separated, the heavier reds cells sink to the bottom and are given back to you. Be sure to provide the laboratory with the amount of plasma specified. Follow the steps in. To ensure adequate mixing, gently invert the BD PPT eight to ten times using a gentle wrist rotation motion. Normal serum (far left) followed by icteric specimens ranging from 1+ to 4+, In all specimens, the normal serum is shown on the left, followed by the abnormal serum specimens; 1) Jaundice/Icterus, 2) Lipemia, 3) Hemolysis; http://clinical-laboratory.blogspot.com/2013/06/preventing-pre-analytical-errors.html. 0000037794 00000 n 0000001651 00000 n Centrifuge Time This prevents the blood from clotting and enables the blood to separate into 3 distinct layers during the centrifugation process. We work witha variety of sample types. If chain of custody documentation is necessary for the procedure, follow the appropriate protocol. A. location such as a hospital room number is not an appropriate patient identifier. When plasma is required, follow these steps. Centrifuge the specimen at the end of the waiting period in accordance with the manufacturer's instructions for speed. When drawing blood, please follow all venipuncture procedures recommended for use by recognized organizations and/or in accordance with applicable state regulations involving phlebotomy practices. 220 mL. (See Figure 12.). Do not centrifuge the specimen for more than 10 minutes unless otherwise specified by the collection instructions. Blood cultures may be ordered for a specific time if a bloodstream bacterial infection is suspected. Mix by inverting specimen with additive immediately after collection. 0000017996 00000 n Plasma Preparation Using a Plasma Preparation Tube (PPT). (See, Submit a quantity of specimen sufficient to perform the test and avoid a QNS (quantity not sufficient), as indicated in the test requirements. 2 0 obj 2023 Laboratory Corporation of America Holdings. These include red blood cells (RBCs), white blood cells, and other components. Hemolysis can be caused by rough handling of a blood specimen, leaving the tourniquet on too long (causing blood stasis) or squeezing the tip of the finger too hard during capillary collection, dilution, exposure to contaminants, extremes in temperature, or pathologic conditions. Handle all slides only by the frosted ends or by the edges. Failure to fill the tube will cause an improper blood-to-anticoagulant ratio and yield questionable and/or QNS test results.

To avoid dietary-induced high lipid levels prior to testing, many physicians require patients to exclude the high-fat foods from their diets or to fast for 12 to 14 hours prior to specimen collection. Collect whole blood in a covered test tube. Adequate patient preparation, specimen collection, and specimen handling are essential prerequisites for accurate test results. Verify the patient's identification. Plastic tubes can be frozen at -80C without risk of breakage. WebIf it turned red colour, we could be explain the hemolysis will occur when animal test. 0000016260 00000 n It is obtained when a clotting-prevention agent is added to whole blood and then placed in a centrifuge to separate the cellular material from the lighter liquid layer. Unless otherwise indicated, serum samples may be sent at room temperature. 0000037578 00000 n The thin portion should be about 1 inch long, and the entire film should cover approximately half of the area of the entire slide. The most common serum preparation considerations: Separate serum from red cells within two hours of venipuncture. Serum-separating tubes (SST) contain a gel and clot activator. WebSpecimens collected in tubes that do not contain a gel separator must be separated after centrifugation by physically removing the supernatant plasma or serum with a pipet and transferring to a plastic aliquot tube. (1983), [2]Inuence of hematocrit on the measurement of lipoproteins demonstrated by the example of lipoprotein(a) Kronenberg F, Trenkwalder M, Kronenberg M, Koenig P, Utermann G and Dieplinger H,Kidney International, 54, 13851389 (1998), [3]Cyclosporin Therapeutic Drug Monitoring an Established Service Revisited, Morris, RG, Clin Biochem Rev 24 33-46 (2003), How Lyophilized Beads Are Revolutionizing Point-of-Care Diagnostics, Science Meets Art in DCN Dx Lateral Flow Training Courses, Faster and More Efficient Detection of Varicella Zoster Virus (VZV) Infections, DCN Dx and Mapp Biopharmaceutical Announce Subcontract, Developing Point-of-Use Diagnostics with Integrated Engineering, PortaCheck, a DCN Dx Brand, Releases New Device for Subclinical Mastitis Detection, Understanding Whole Blood, Serum, and Plasma, DCN Dx Acquires Microbiological Testing Products Company Biomed Diagnostics, DCN Dx To Host Career Fair and Open House, DCN Dx Partners with Mainz Biomed on Clinical Studies for Colorectal Cancer Product, Blood Collection/Separation Materials Kit. Prepare the needle holder in order to attach the safety needle in the appropriate manner. This is necessary because RBCs can rupture over time, and the contents can interfere with a number of assays. This usually takes 1530 minutes. Turn the centrifuge off, if not automatic turn off, and allow it to come to a complete stop. 0000001794 00000 n Draw whole blood in an amount 2 times the required volume of serum so that a sufficient amount of serum can be obtained. It is quick and easy to get excellent separation of centrifuged blood with the aid of a high-quality blood separation centrifuge such as the CAPPRondo Advanced Clinical Centrifuge CRC-416X. Patients who consume these foods within the 24-hour period immediately preceding collection of a blood specimen may have temporarily elevated lipid levels, which may be manifested by cloudy or lipemic serum. You can also go the test description for Blood Culture, Routine [008300] in LabCorp's online directory and refer to the Microbiology Specimen Collection and Transport Guide attached in the Related Documents field for additional information on blood culture specimen collection. Blood Specimens: Chemistry and Hematology Preparing Serum, Combatting Modern Slavery and Human Trafficking Statement, Please examine specimen collection and transportation supplies to be sure they do not include, Label a specimen correctly and provide all pertinent information required on the test request form. This is performed by centrifuging your blood (spinning it down) at a high rate of speed (rounds per minute or rpms) in a centrifuge.